The project studies energy expenditure and physical activity in American Samoan children, with the eventual goal of understanding if differences in children's activity levels have an impact on obesity trends in adulthood. This summer, we will conduct an abbreviated version of the study on a small sample of 30-40 children to ensure that we can effectively measure their energy expenditure and activity levels. We aren't concerned so much with random sampling or the actual analysis of the data so much as how well the data collection goes. For each participant, we will measure some basic anthropometric traits (height, weight, blood pressure, etc), carry out a doubly labeled water test, administer an activity monitor, and collect information on dietary intake. The activity monitor and doubly labeled water methods will be the two under the closest scrutiny.
The doubly labeled water (DLW) method involves giving the children a sample of water with two heavy isotopes (O18 and H2) and taking six urine samples over the course of 7 days. We will submit the samples to a mass spectrometry lab, which will give us the total energy expenditure for each participant. Using height and weight, we will estimate resting energy expenditure (the amount of energy your body uses just to stay alive) and subtract it from the total energy expenditure to get energy expenditure due to physical activity. This method is fairly commonly used now, but we must verify that it will work specifically among American Samoan children. Click Here for a more detailed description of DLW.
The project will certainly keep me busy for the summer. Even with five of us working, we will be following nearly 40 children for 7 days each - no easy task. However, working directly with the children should be both fun and educational, a great combination for a summer research project. Even though everything sounds very systematic, the realities of recruiting kids and getting them to go along with our crazy research endeavors will likely prove much more chaotic. I guess I'll know soon enough.
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